Effect of Yishenqinghuo compound on biological characteristics of rat bone marrow stromal cells / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the effect of Yishenqinghuo recipe on biological characteristic of rat bone marrow stromal cells (BMSCs) and search for the function and mechanism of this recipe in treatment of periodontitis.</p><p><b>METHODS</b>12- to 15-month-old SD rats were allocated into 5 groups. Group A control group (with no periodontitis model, fed with the same dosage of saline as Group D); Group B model group (with periodontitis model, fed with the same dosage of saline as Group D); Group C high dosage group (with periodontitis model, fed with a high dosage of medicine); Group D middle dosage group (with periodontitis model, fed with a middle dosage of medicine); and Group E low dosage group (with periodontitis model, fed with a low dosage of medicine). Ten days later, serums were collected from all the five groups for in vitro cultivation of BMSCs.</p><p><b>RESULTS</b>There was displayed a similarity in effect between the serum collected from Group C1 (serum collected 0.5 h after the last gavage of Group C) and that from Group A after the last gavage, the effect being the best in terms of proliferation of BMSCs. A comparison with the other groups had revealed a striking difference (P < 0.01), with Group B having turned out to be the worst. The serum from Group C2 (serum collected 1 h after the last gavage of Group C) after the last gavage could best enhance the generation and activity of ALP, having demonstrated a significant difference (P < 0.01) in comparison with the other groups.</p><p><b>CONCLUSION</b>Yishenqinghuo recipe can improve the proliferation of BMSCs and facilitate the differentiation to osteoblast.</p>