Integrin 3 mRNA changes after orthodontic teeth movement in periodontitis rats / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the integrin beta3 mRNA changes after orthodontic treatment on normal teeth and periodontitis teeth in rats.</p><p><b>METHODS</b>96 adult SD rats of 10 weeks old were randomly divided into normal tooth move-ment group and periodontitis tooth movement group. The rats in the two groups were sacrificed after 0 d, 12 h, 1 d, 3 d, 5 d and 7 d of tooth movement. The alveolar specimens were prepared. The integrin beta3 mRNA were detected using in situ hybridization in the specimens. The OD index of positively stained osteoclasts for integrin beta3 mRNA after orthodontic tooth movement in the two groups were measured and compared.</p><p><b>RESULTS</b>There were weak positive signals on the cytoplasm of osteoclasts in periodontum in both groups after 12 hours and 3 days force activation. No positive signals were detected in the rest samples. There was no difference in the OD of positive stained osteoclasts between normal and periodontitis groups. Strong expressions were present on cells with one or two nuclei in the alveolar marrow.</p><p><b>CONCLUSION</b>It is suggested that integrin beta3 mRNA is related with osteoclasts maturation and migration in orthodontic tooth movement.</p>