Characteristics of free Ca2+ distribution in cultured osteoclast-like cells / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the spatial distribution of free Ca2+ in osteoclast-like cells cultured on glass.</p><p><b>METHODS</b>To detect the free Ca2+ in osteoclast-like cells, the images were analyzed with image software, using the laser scanning confocal microscope and fluorescent probe.</p><p><b>RESULTS</b>At 37 degrees C the free Ca2+ in osteoclast-like cells could be labelled effectively with 10 micromol/L Fluo-3/AM, the intensity of Ca2+ fluorescent signal in the central part was greater than that in the peripheral part and in the same section the signal was not distributed evenly.</p><p><b>CONCLUSION</b>The intensity of Ca2+ fluorescent signal is different among various organellae in osteoclast-like cell, which suggests the osteoclast-like cell modulate its own function through the spatial difference of free Ca2+ concentration.</p>