Preparation of enamel matrix proteins controlled release microspheres and their biological effects on the proliferation and differentiation of human periodontal ligament cells in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 529-533, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-289023
ABSTRACT
<p><b>OBJECTIVE</b>To prepare enamel matrix proteins (EMPs) loaded dextran-based hydrogel microspheres (EMPs-dex-MPs), and to evaluate their EMPs controlled release property and their biological effects on the proliferation and differentiation of human periodontal ligament cells (PDLCs) in vitro.</p><p><b>METHODS</b>Using dimethylbenzene as the oil phase, EMPs-dex-MPs were achieved by emulsion-chemical crosslinking technique. The process of the recombination preparation was optimized by orthogonal factorization method. The configuration and size of EMPs-dex-MPs were determined by scanning electron microscope. The EMPs loading content and encapsulation rate of EMPs-dex-MPs, and their biodegradation characteristic were studied by routine analysis methods. Dynamic dialysis method was used to determine the release characteristic of EMPs-dex-MPs in vitro and its influencing factors. The proliferation of cultured PDLCs was measured by MTF method and the differentiation of PDLCs was measured by their alkaline phosphatase (ALP) activities.</p><p><b>RESULTS</b>The results showed that EMPs-dex-MPs were homogenous and stable with the average diameter 25 microm, and the EMPs loading content was (32.8 +/- 1.2)%, the encapsulation rate was (78.9 +/- 1.0)%. Under 9% physiological saline solution contained a very thimbleful quantity of dextranase EMPs-dex-MPs could be biodegraded completely during about 40 days. The in vitro experiments showed that about 80% of EMPs could be released out in 20 days. Using EMPs-dex-MPs could enhance the proliferation responses and ALP activities of PDLCs more than 12 days.</p><p><b>CONCLUSION</b>As a new sustained release system of growth factors, the dex-MPs is stable, workable and biodegradable. EMPs-dex-MPs, whose drug release can be controlled by preparation technique, may be more effective in promoting periodontal tissue regeneration.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ligamento Periodontal
/
Regeneración
/
Técnicas In Vitro
/
Diferenciación Celular
/
Células Cultivadas
/
Dextranos
/
Preparaciones de Acción Retardada
/
Proteínas del Esmalte Dental
/
Microesferas
Límite:
Humanos
Idioma:
Chino
Revista:
West China Journal of Stomatology
Año:
2005
Tipo del documento:
Artículo
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