Immortalized human odontoblast-like cell line expressed dentin extracellular matrix in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 518-521, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-289026
ABSTRACT
<p><b>OBJECTIVE</b>To determine whether dentin matrix proteins were expressed by the human odontoblast-like cell line hTERT-hOd-1 in vitro.</p><p><b>METHODS</b>Collagen type I, bone sialoprotein (BSP), dentin matrix protein 1 (DMP1) and the marker for odontoblast, dentin sialophosphoprotein (DSPP) and dentin sialoprotein (DSP) were detected in these cells by immunohistochemistry, RT-PCR and in situ hybridization. During being cultured in mineralizing medium for 5 weeks, the secretion of OC and activity of ALP were measured once a week.</p><p><b>RESULTS</b>DSPP, DMP1, BSP and collagen type I were expressed in hTERT-hOd-1 either at mRNA or protein level. Under the induction of mineralizing medium, the cells showed higher activity of ALP and increased secretion of OC.</p><p><b>CONCLUSION</b>hTERT-hOd-1 expressed dentin extracellular matrix in vitro, which means the cell line has the potential of mineralization.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosfoproteínas
/
Sialoglicoproteínas
/
Técnicas In Vitro
/
ARN Mensajero
/
Inmunohistoquímica
/
Línea Celular
/
Proteínas de la Matriz Extracelular
/
Hibridación in Situ
/
Colágeno Tipo I
/
Dentina
Límite:
Humanos
Idioma:
Chino
Revista:
West China Journal of Stomatology
Año:
2005
Tipo del documento:
Artículo
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