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RNA interference inhibits VEGF expression and growth of PC-3 in prostate carcinoma / 中华男科学杂志
National Journal of Andrology ; (12): 1095-1098, 2006.
Artículo en Chino | WPRIM | ID: wpr-289071
ABSTRACT
<p><b>OBJECTIVE</b>To construct a eukaryotic expression vector carrying human VEGF RNAi and to study the effect of RNA interference on VEGF expression in prostate carcinoma.</p><p><b>METHODS</b>VEGF RNAi was synthesized, inserted into the RNA interference eukaryotic expression vector, and confirmed by the result sequencing. The vector was transfected into prostate cancer PC-3, the VEGF expression detected by Western blot and the cell inhibiting rate determined by MTT.</p><p><b>RESULTS</b>The VEGF RNAi eukaryotic expression vector was successfully constructed. Compared with the empty vector group and the control group, the amount of VEGF protein expression was obviously decreased in the VEGF RNAi group. The inhibiting rates were 23.5% , 33. 5% and 40. 8% at 24, 48 and 72 h respectively.</p><p><b>CONCLUSION</b>VEGF RNAi can inhibit the protein expression and growth of PC-3, which provides an experimental base for the biological therapy of prostate cancer.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Neoplasias de la Próstata / Transfección / Expresión Génica / Interferencia de ARN / Línea Celular Tumoral / Factor A de Crecimiento Endotelial Vascular / Genética / Metabolismo / Neoplasias Hormono-Dependientes Límite: Humanos / Masculino Idioma: Chino Revista: National Journal of Andrology Año: 2006 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Neoplasias de la Próstata / Transfección / Expresión Génica / Interferencia de ARN / Línea Celular Tumoral / Factor A de Crecimiento Endotelial Vascular / Genética / Metabolismo / Neoplasias Hormono-Dependientes Límite: Humanos / Masculino Idioma: Chino Revista: National Journal of Andrology Año: 2006 Tipo del documento: Artículo