Type IV collagenase expression in human prostate carcinoma cell lines with different metastasis potentials / 中华男科学杂志
National Journal of Andrology
;
(12): 913-916, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-289110
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the correlation between type IV collagenase expression and metastatic potential of human prostate carcinoma cells.</p><p><b>METHODS</b>Human prostate carcinoma cell lines (LNCaP and C4-2B) with different metastatic potentials were selected. Using SABC immunocytochemical staining and zymographic analysis, we detected the difference of type IV collagenase expression and activity between the two cell lines.</p><p><b>RESULTS</b>The expression of type IV collagenase (MMP-2, MMP-9) could be detected in both LNCaP and C4-2B, mainly in cytoplasm, much higher in C4-2B than in LNCaP (P < 0.01). The activities of type IV collagenase in the conditioned medium of prostatic carcinoma cell LNCaP was the lowest, and the quantitative estimation values of MMP-2 and MMP-9 were 0.89 +/- 0.02 and 0.86 +/- 0.01, respectively. However, the quantitative estimation values of MMP-2 and MMP-9 in the conditioned medium of C4-2B were 96.32 +/- 4.36 and 33.89 +/- 1.84, respectively. Compared with LNCaP, the activity of type IV collagenase in C4-2B was much higher (P < 0.01).</p><p><b>CONCLUSION</b>Compared with LNCaP, the higher production capability of type IV collagenase in C4-2B may possibly contribute to its invasive and metastatic potentials. The expression of type IV collagenase of human prostate carcinoma cells is closely correlated to the metastatic and invasive potential.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Neoplasias de la Próstata
/
Metaloproteinasa 2 de la Matriz
/
Metaloproteinasa 9 de la Matriz
/
Línea Celular Tumoral
/
Metabolismo
/
Invasividad Neoplásica
/
Metástasis de la Neoplasia
Límite:
Humanos
/
Masculino
Idioma:
Chino
Revista:
National Journal of Andrology
Año:
2006
Tipo del documento:
Artículo
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