AFLP analysis of genetic diversity of Alpinia officinarum / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the genetic diversity and relationship of different Alpinia officinarum germplasm.</p><p><b>METHOD</b>Amplified fragment length polymorphism (AFLP) markers were developed to analyze genetic polymorphism in A. officinarun from eight resources. The amplified fragments were used as primary matrix with NTSYSpc-2.11F software to analyze the similarity between the A. officinarum germplasm and to construct the genetic phylogenetic tree.</p><p><b>RESULT</b>A total of 1,120 fragments were genotyped using AFLP with eight prime combinations. Analysis identified 1,044 polymorphic fragments, accounting for 92.57% of the total detected variation. Genetic phylogenetic tree analysis indicates that three categories can be divided among the eight resources of A. officinanrum.</p><p><b>CONCLUSION</b>Significant polymorphism and genetic diversity can be observed among A. officinarum germplasm resources.</p>