Classification of Pueraria lobata in different geographical regions / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To classify Pueraria lobata originated from different geographical regions based on ITS,psbK-psbI and trnH-psbA information.</p><p><b>METHOD</b>Twenty-four samples of P. lobata were collected from northeast China, north China, central China and northwest China. DNA extraction, PCR, sequence and genotypes/haplotypes analysis were performed .</p><p><b>RESULT</b>ITS1, 5.8S and ITS2 varied only 1 bp respectively, psbK-psbI 2 bps; trnH-psbA varied 1 bp and 10 bp deletion.</p><p><b>CONCLUSION</b>Based on the variation of ITS,psbK-psbI and trnH-psbA, 4 genotypes and 2 haplotypes were identified, respectively.</p>