Instantaneous expression aFGF-GFP fusion gene in safflower / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 281-284, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-289384
ABSTRACT
<p><b>OBJECTIVE</b>To study the instantaneous expression aFGF-GFP fusion gene in Carthamus tinctorius.</p><p><b>METHOD</b>Molecular biology methods were applied to construct aFGF and GFP fusion gene vector, it is transformed into C. tinctorius by Agrobacterium tumefaciens, forming the resistant callus, fluorescence microscopy was used for detection.</p><p><b>RESULT</b>aFGF gene and GFP gene were amplified by PCR reaction. It was successfully constructed plant fluorescence expression vector pCAMBIA139035SaFGF-GFP, it was used to transform C. tinctorius, and the acquired resistance calli showed strong green fluorescence under UV light.</p><p><b>CONCLUSION</b>The expression of GFP in resistance C. tinrictorius calli is good, it is indicated that aFGF gene in plant cells has also been expressed.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Recombinantes de Fusión
/
Expresión Génica
/
Clonación Molecular
/
Carthamus tinctorius
/
Proteínas Fluorescentes Verdes
/
Factores de Crecimiento de Fibroblastos
/
Genética
/
Metabolismo
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2011
Tipo del documento:
Artículo
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