Effect of Endometrial Stem Cell-derived Cytokine Cocktail on a Mouse Model of Myocardial Reperfusion Injury / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 253-259, 2016.
Artículo
en Inglés
| WPRIM
| ID: wpr-289874
ABSTRACT
Objective To study the effect of endometrial stem cells (EnSCs) derived cytokine cocktail (EdCC) on myocardial ischemic reperfusion injury (I/R) in a mouse model. Methods EdCC was concentrated from the culture medium of EnSCs with Millipore ultra-filtration technology and was administrated to a myocardial I/R mouse models through tail vein injection. The infarct area was determined by TTC/Evans Blue staining. The apoptotic cells were counted by TUNEL assay and the protein level of cleaved caspase 3 was evaluated by Western blotting. Results The EdCC extraction efficiency was (222.4±29.3) Μg/10(6) cells in every 24 h,but the protein gradually degraded under-80 ℃ storage. As compared with I/R group,100 Μg fresh EdCC decreased infarct area (P=0.001),reduced apoptotic nuclei in the infarct border (P=0.019),and inhibited cleaved caspase 3 expression (P=0.002). Increasing EdCC dosage did not further reduce the infarct area. The myocardial protective effect of EdCC diminished after 90 days' storage under-80 ℃. Conclusion EdCC reduces myocardial I/R injury through protecting cardiomyocytes from apoptosis within 30 days storage under-80 ℃.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células Madre
/
Terapéutica
/
Daño por Reperfusión Miocárdica
/
Células Cultivadas
/
Citocinas
/
Apoptosis
/
Biología Celular
/
Miocitos Cardíacos
/
Usos Terapéuticos
/
Modelos Animales de Enfermedad
Límite:
Animales
Idioma:
Inglés
Revista:
Acta Academiae Medicinae Sinicae
Año:
2016
Tipo del documento:
Artículo
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