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Investigation on vibrio cholera carried in aquatic products of littoral areas, Zhejiang Province / 中华预防医学杂志
Chinese Journal of Preventive Medicine ; (12): 336-338, 2006.
Artículo en Chino | WPRIM | ID: wpr-290263
ABSTRACT
<p><b>OBJECTIVE</b>To examine vibrio cholera (V.C) in aquatic products of littoral area, Zhejiang Province and to provide scientific evidence for administration of aquatic products and cholera epidemic control.</p><p><b>METHODS</b>All 990 samples of aquatic products collected from local markets, eateries and aquafarms in three chosen areas. Samples were proliferated in alkaline liquid medium, and purified in NO 4 medium, the isolations were identified biochemically, and phenotype of strains were defined by phagocyte and coagulation with V.C. diagnostic serum. Three virulence genes (ctx, ace, zct) of the isolated strains were detected by polymerase chain reaction (PCR).</p><p><b>RESULTS</b>There were 1.41% samples caught by V.C., having a carrying rate highest in turtles of 8.9%. 14 strains were defined as three serogroups, and the numbers of Inaba, Ogawa, and Hikojima types were 2, 2, 10 respectively. Virulence genes had detected in 9 of 12 stains. All genes were detected in 5 strains, only ZOT genes in 3 strains, and both CTX and ACE genes in 1 strain.</p><p><b>CONCLUSIONS</b>Aquatic products from inshore in Zhejiang Province caught with V.C. strains might be divided into three serogroups. Most of them should be virulence genes. Cholera epidemic outbreak might be caused by those contaminated products.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Vibrio cholerae / China / Alimentos Marinos / Factores de Virulencia / Microbiología de Alimentos / Genes Bacterianos / Genética / Microbiología Tipo de estudio: Estudio pronóstico País/Región como asunto: Asia Idioma: Chino Revista: Chinese Journal of Preventive Medicine Año: 2006 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Vibrio cholerae / China / Alimentos Marinos / Factores de Virulencia / Microbiología de Alimentos / Genes Bacterianos / Genética / Microbiología Tipo de estudio: Estudio pronóstico País/Región como asunto: Asia Idioma: Chino Revista: Chinese Journal of Preventive Medicine Año: 2006 Tipo del documento: Artículo