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Effects of arecoline on calcium channel currents and caffeine-induced calcium release in isolated single ventricular myocyte of guinea pig / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 279-287, 2002.
Artículo en Inglés | WPRIM | ID: wpr-290535
ABSTRACT
The effects of Arecoline (Are) on calcium mobilization were investigated. In isolated single ventricular myocyte of guinea pig, patch clamp whole cell recording techniques were used to record the current of L-type calcium channel and cytosolic Ca2+ level ([Ca2+]i) labeled with fluorescence probe Fluo-3/AM was measured under a laser scanning confocal microscope. Results revealed that Are (3-100 mumol/L) could inhibit L-type calcium current in a concentration-dependent manner and the value of IC50 was 33.73 mumol/L (n = 5). In the absence of extracellular calcium, the resting levels of [Ca2+]i was not affected by Are (n = 6, P > 0.05), but pretreatment with Are (30 mumol/L) could significantly inhibit the [Ca2+]i elevation induced by caffeine (10 mmol/L, n = 6, P < 0.01). It was concluded that Are could inhibit not only calcium influx through L-type calcium channel but also calcium release from sarcoplasmic reticulum.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Arecolina / Retículo Sarcoplasmático / Transporte Biológico Activo / Cafeína / Separación Celular / Calcio / Técnicas de Placa-Clamp / Agonistas Colinérgicos / Canales de Calcio Tipo L Límite: Animales Idioma: Inglés Revista: Journal of Huazhong University of Science and Technology (Medical Sciences) Año: 2002 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Arecolina / Retículo Sarcoplasmático / Transporte Biológico Activo / Cafeína / Separación Celular / Calcio / Técnicas de Placa-Clamp / Agonistas Colinérgicos / Canales de Calcio Tipo L Límite: Animales Idioma: Inglés Revista: Journal of Huazhong University of Science and Technology (Medical Sciences) Año: 2002 Tipo del documento: Artículo