Effects of Survivin-T34A mutant on breast cancer cell in vitro and in vivo / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 648-651, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-290699
ABSTRACT
To study the effect of the proliferation and apoptosis of Survivin-T34A mutant on breast cancer MCF-7 cell, we adopted the method of cell culture in vitro to observe the proliferation and apoptosis of the cell. In the experiment, MCF-7 cells were randomly divided into three groups and transfected with normal saline, PORF-9-null and Survivin-T34A, respectively. Breast cancer nude mouse models were established to study anti-tumor effect of Survivin-T34A in vivo. The activity of the cells in the Survivin-T34A-transfected group was lower than that in PORF-9-null group. The increase of cell apoptosis was observed under electron microscopy, meanwhile the apoptotic rate was obviously higher than that in PORF-9-null control by flow cytometry. Tumor inhibition effects of the mouse which received the injection of Survivin-T34A intratumoral injection were apparent, and the inhibition ratio was as high as 47.1%. In conclusion, Survivin-T34A mutant has anti-tumor effect through efficiently inhibiting the growth of breast cancer MCF-7 cell and actively promoting apoptosis of cancer cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Neoplasias de la Mama
/
Transfección
/
Inyecciones Intralesiones
/
Apoptosis
/
Quimioterapia
/
Proteínas Inhibidoras de la Apoptosis
/
Genética
/
Ratones Desnudos
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Journal of Biomedical Engineering
Año:
2014
Tipo del documento:
Artículo
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