The rescue effect of RANKL on zoledronate induced acid inhibition of osteoclastogenesis and gene expression of NF-kappaB p50 and c-Jun / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 385-399, 2014.
Article
en Zh
| WPRIM
| ID: wpr-290748
Biblioteca responsable:
WPRO
ABSTRACT
In this study, the rescue effect of receptor activator for nuclear factor-kappaB ligand (RANKL) on zoledronate acid (ZOL) induced inhibition of osteoclastogenesis and gene expression of NF-kappaB p50 and c-Jun was investigated. Mice calvarial osteoblasts (OBs) were harvested and co-cultured with RAW264.7 cells and the cells were divided into 4 groups and received treatment with ZOL and RANKL, either single or combined. The formation of multi-nucleated osteoclast (OC) was examined and gene expression of NF-kappaB p50 and c-Jun was detected. Group B (ZOL) showed least multi-nucleated OC and resorption lacunae among the 4 groups (P < 0.05 or P < 0.01) and it was followed by group C (ZOL+RANKL). Group D (RANKL) showed highest OC and resorption lacunae while it was similar to Group A (control) (P > 0.05). Gene expression of NF-kappaB p50 and c-Jun was the lowest in group B (P < 0.05 or P < 0.01) among the four groups and was significantly increased in group C when compared with group B (P < 0.05). Group A and D showed highest gene expression and they were similar to each other (P > 0.05). This study suggest that RANKL might partly rescue ZOL induced inhibition of osteoclastogenesis, and the effect of RANKL and ZOL on osteoclastogenesis may be mediated by NF-kappaB p50 and c-Jun.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Osteoblastos
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Osteoclastos
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Farmacología
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Resorción Ósea
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Expresión Génica
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Línea Celular
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Proteínas Proto-Oncogénicas c-jun
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Difosfonatos
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Quimioterapia
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Subunidad p50 de NF-kappa B
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Año:
2014
Tipo del documento:
Article