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Anti-tumor effect of adenovirus-mediated Bcl-XL shRNA in vitro / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery ; (12): 292-294, 2012.
Artículo en Chino | WPRIM | ID: wpr-290799
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-tumor effect of adenovirus-mediated Bcl-XL shRNA on colon cancer cells in vitro.</p><p><b>METHODS</b>A recombinant Bcl-xl adenovirus was constructed, amplified, and purified. The effect on mRNA expression of Bcl-XL was assessed by RT-PCR, and the effect on apoptosis-induction of colon cancer(Lovo cell line) in vitro was assessed by MTT assay and cell clonogenic assay.</p><p><b>RESULTS</b>RT-PCR showed that Ad/Bcl-XL shRNA significantly down-regulated the mRNA expression of Bcl-XL in Lovo cells. Ad/Bcl-XL shRNA suppressed the proliferation of Lovo cells in a dose-dependent as well as a time-dependent manner compared with Ad/GFP (P<0.05). Treatment with Ad/Bcl-XL shRNA dramatically suppressed the colony formation of Lovo cells in a dose-dependent manner (P<0.05). Ad/Bcl-XL shRNA showed no effect on normal human fibroblast.</p><p><b>CONCLUSION</b>Ad/Bcl-XL shRNA exhibits cytotoxic effect on Lovo cells and may have the potential value in the treatment of colon cancer.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Adenoviridae / Neoplasias del Colon / ARN Interferente Pequeño / Línea Celular Tumoral / Proliferación Celular / Proteína bcl-X / Genética / Metabolismo Límite: Humanos Idioma: Chino Revista: Chinese Journal of Gastrointestinal Surgery Año: 2012 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Adenoviridae / Neoplasias del Colon / ARN Interferente Pequeño / Línea Celular Tumoral / Proliferación Celular / Proteína bcl-X / Genética / Metabolismo Límite: Humanos Idioma: Chino Revista: Chinese Journal of Gastrointestinal Surgery Año: 2012 Tipo del documento: Artículo