Anti-tumor effect of adenovirus-mediated Bcl-XL shRNA in vitro / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 292-294, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-290799
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-tumor effect of adenovirus-mediated Bcl-XL shRNA on colon cancer cells in vitro.</p><p><b>METHODS</b>A recombinant Bcl-xl adenovirus was constructed, amplified, and purified. The effect on mRNA expression of Bcl-XL was assessed by RT-PCR, and the effect on apoptosis-induction of colon cancer(Lovo cell line) in vitro was assessed by MTT assay and cell clonogenic assay.</p><p><b>RESULTS</b>RT-PCR showed that Ad/Bcl-XL shRNA significantly down-regulated the mRNA expression of Bcl-XL in Lovo cells. Ad/Bcl-XL shRNA suppressed the proliferation of Lovo cells in a dose-dependent as well as a time-dependent manner compared with Ad/GFP (P<0.05). Treatment with Ad/Bcl-XL shRNA dramatically suppressed the colony formation of Lovo cells in a dose-dependent manner (P<0.05). Ad/Bcl-XL shRNA showed no effect on normal human fibroblast.</p><p><b>CONCLUSION</b>Ad/Bcl-XL shRNA exhibits cytotoxic effect on Lovo cells and may have the potential value in the treatment of colon cancer.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Adenoviridae
/
Neoplasias del Colon
/
ARN Interferente Pequeño
/
Línea Celular Tumoral
/
Proliferación Celular
/
Proteína bcl-X
/
Genética
/
Metabolismo
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Gastrointestinal Surgery
Año:
2012
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS