Presynaptic alpha-7 nicotinic acetylcholine receptors modulate excitatory synaptic transmission in hippocampal neurons / 生理学报
Acta Physiologica Sinica
;
(6): 731-735, 2003.
Artículo
en Inglés
| WPRIM
| ID: wpr-290900
ABSTRACT
The effects of presynaptic nicotinic acetylcholine receptors (nAChRs) on excitatory synaptic transmission in CA1 pyramidal neurons of the rat hippocampus were examined by blind whole-cell patch clamp recording from hippocampal slice preparations. Local application of the nAChRs agonist dimethylphenyl-piperazinium iodide (DMPP) did not induce a postsynaptic current response in CA1 pyramidal cells. However, DMPP enhanced the frequency and amplitude of spontaneous excitatory postsynaptic current (sEPSC) in these cells in a dose-dependent manner. This enhancement was blocked by the selective nicotinic alpha-7 receptor antagonist alpha-bungarotoxin, but not by the antagonist mecamylamine, hexamethonium or dihydro-beta-erythroidine. The frequency of miniature excitatory postsynaptic current (mEPSC) in CA1 pyramidal neurons was also increased by application of DMPP, indicating a presynaptic site of action of the agonist. Taken together, these results suggest that activation of presynaptic nAChRs in CA1 pyramidal neurons, which contain alpha-7 subunits, potentiates presynaptic glutamate release and consequently modulate excitatory synaptic transmission in the hippocampus.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Marcapaso Artificial
/
Farmacología
/
Fisiología
/
Sinapsis
/
Bungarotoxinas
/
Receptores Nicotínicos
/
Ratas Wistar
/
Receptores Presinapticos
/
Transmisión Sináptica
/
Técnicas de Placa-Clamp
Límite:
Animales
Idioma:
Inglés
Revista:
Acta Physiologica Sinica
Año:
2003
Tipo del documento:
Artículo
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