CD226 monoclonal antibody induces variation of intracytosolic free calcium level in human umbilical vein endothelial cells / 生理学报
Acta Physiologica Sinica
;
(6): 355-359, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-290960
ABSTRACT
In order to study the possible mechanism of CD226 monoclonal antibody (mAb)-mediated intracellular message transduction in human umbilical vein endothelial cells (HUVECs), the influence of CD226 mAb and its cross-linking by secondary antibody (II Ab) on the concentration changes in [Ca(2+)](i) in the HUVECs under different conditions were determined by confocal laser scanning microscopy. The main results are as follows. (1) When the culture medium was balanced by Hanks Buffer, [Ca(2+)](i) in HUVECs increased slowly after stimulation by CD226 mAb, whereas [Ca(2+)](i) increase was accompanied by [Ca(2+)](o) decrease after the mAb was cross-linked by goat anti-mouse IgG. Then [Ca(2+)](i) and [Ca(2+)](o) all returned to the normal level. (2) When the culture medium was balanced by D-Hanks buffer, [Ca(2+)](i) in HUVECs showed little variation when the cells were stimulated by CD226 mAb, but [Ca(2+)](i) decreased markedly after cross-linking. (3) When HUVECs were pretreated with EGTA, there was no variation in [Ca(2+)](i) of HUVECs after CD226 mAb stimulation alone or cross-linking of the mAb. Our results suggest that stimulation by CD226 mAb and cross-linking by goat anti-mouse IgG induce the variation of [Ca(2+)](i) in HUVECs under different conditions and the variation of [Ca(2+)](i) in HUVECs may play an important role in many physiological and pathological processes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fisiología
/
Antígenos de Diferenciación de Linfocitos T
/
Células Cultivadas
/
Calcio
/
Alergia e Inmunología
/
Células Endoteliales de la Vena Umbilical Humana
/
Metabolismo
/
Anticuerpos Monoclonales
Límite:
Humanos
Idioma:
Chino
Revista:
Acta Physiologica Sinica
Año:
2003
Tipo del documento:
Artículo
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