Gene expression profile of yolk sac and fetal liver in mouse / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 266-268, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-291452
ABSTRACT
<p><b>OBJECTIVE</b>To better understand the mechanisms of the fetal hematopoiesis turn over from primitive to definitive hematopoiesis through the expression level of c-kit(+) and sca-1(+), and major characters of gene expression profile of these cells.</p><p><b>METHODS</b>c-kit and sca-1 expression level were monitored with fluorescence activated cell sorting (FACS) of the mononuclear cells from mouse yolk sac and fetal liver, while gene expression profile was carried out with EST sequencing strategy.</p><p><b>RESULTS</b>The Sca-1(+) cells were increased while the c-kit(+) cells decreased with the embryonic development. Through profiling the functionally identified known genes, most of the highly expressed were globin genes, especially of embryonic types.</p><p><b>CONCLUSION</b>The erythropoiesis played a key role in early fetal hematopoiesis in mammalian.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Factores de Tiempo
/
Saco Vitelino
/
Antígenos Ly
/
Diferenciación Celular
/
Embriología
/
Regulación del Desarrollo de la Expresión Génica
/
Proteínas Proto-Oncogénicas c-kit
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Perfilación de la Expresión Génica
/
Biología Celular
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Hematology
Año:
2004
Tipo del documento:
Artículo
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