Identification of a novel HLA allele HLA-DRB1*09:01:07 / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To identify and confirm a novel HLA allele in a Chinese Han individual.</p><p><b>METHODS</b>HLA typing was performed by polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) for HLA-A, -B and -DRB1 in a registered donor of China Marrow Donor Program(CMDP). Sequencing-based typing (SBT) was carried out to further confirm the novel allele of HLA-DRB1.</p><p><b>RESULTS</b>The SSOP result showed HLA-DRB1*09:03,15GEP, but an unusual pattern that could not be defined indicated potential presence of a novel allele. The SBT result showed the novel sequence has 1nt change from its closest allele DRB1*09:01:02 at nt306 where C to T(codon 73 GCC to GCT) resulting in no amino acid change. 73A was not changed.</p><p><b>CONCLUSION</b>A novel HLA allele, HLA-DRB1*09:01:07, has been identified and named officially by WHO Nomenclature Committee for Factors of the HLA System.</p>