Confirmation of 17 rare HLA alleles and prediction of their haplotypes / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To confirm 17 rare HLA alleles detected during routine HLA typing and deduce their haplotypes.</p><p><b>METHODS</b>Bi-allelic sequence-based typing and Luminex DNA PCR-SSOP assay were applied for the initial or repeat HLA typing, respectively. The rare HLA alleles were confirmed with mono-allelic sequence-based typing. Predicted haplotypes of the rare alleles were inferred based on the frequencies of HLA alleles and haplotypes in Han population.</p><p><b>RESULTS</b>The authenticity of the total 17 rare HLA alleles was proven, and 18 predicted haplotypes associated with the rare alleles were recognized. A*1112 and DRB1*1319 were detected twice among unrelated individuals.</p><p><b>CONCLUSION</b>Study of rare HLA alleles and predicted haplotype can provide useful information for donor searching and transplantation, and enrich polymorphisms of HLA in this population.</p>