Role of telomerase in chrysotile induced malignant transformation of normal human embryonic lung fibroblasts / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 167-171, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-291797
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of telomerase in asbestos dust induced malignant transformation of human embryonic lung fibroblasts in vitro.</p><p><b>METHODS</b>Human telomerase catalytic subunit (hTERT) was transferred into human embryonic lung fibroblasts (HELF). Chrysotile dust at concentration of 2.5 microg/cm(2) was added to HELF transduced with and without hTERT (HELF-T+), respectively, and their transduced foci were separated. Biological characteristics of the cells, telomerase activity, length of telomere and cell growth curve were observed. Colony forming test was performed on soft agar to evaluate the nature of transformation.</p><p><b>RESULTS</b>The hTERT gene was transferred into HELF steadily, and HELF-T+ was established. Malignant transformation occurred in both HELF and HELF-T+ by asbestos stimulation. Asbestos dusts could induce higher rate of transformations in HELF-T+ [(2.08 +/- 1.08)/utensil] than in HELF [(1.08 +/- 0.10)/utensil], P < 0.05. Telomerase activity in both transformed malignant cells and HELF-T+ was higher, as well as the longer length of telomere in them.</p><p><b>CONCLUSION</b>Rate of malignant transformation in cells with more activity of telomerase and longer length of the telomere was higher after stimulation with asbestos, indicating telomerase could play an important role in asbestos induced human cells malignant transformation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Transformación Celular Neoplásica
/
Células Cultivadas
/
Técnicas de Transferencia de Gen
/
Asbestos Serpentinas
/
Telomerasa
/
Proteínas de Unión al ADN
/
Embrión de Mamíferos
/
Toxicidad
/
Fibroblastos
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Preventive Medicine
Año:
2004
Tipo del documento:
Artículo
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