Effects of timely insulin treatment on protection of beta cells in a rat model of type 2 diabetes mellitus / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1523-1529, 2004.
Artículo
en Inglés
| WPRIM
| ID: wpr-291887
ABSTRACT
<p><b>BACKGROUND</b>Insulin treatment plays a key role in management of diabetes mellitus. Clinical researches showed that extra improvements in restoration of insulin secretion of pancreatic beta cells were found in patients with newly diagnosed type 2 diabetes. The purpose of this study was to investigate the effects of early insulin treatment on insulin mRNA expression and morphological alterations of beta cells in a Sprague Dawley (SD) rat model of type 2 diabetes.</p><p><b>METHODS</b>A rat model of type 2 diabetes mellitus (T2DM) was induced by a high fat diet (high energy, HE) and low doses of streptozotoxin (STZ, 40 mg/kg). A group of diabetic rats was then injected with protamine zinc insulin [PZI, 1 - 2 U x kg(-1) x d(-1)] for one week. Insulin mRNA expression, morphological features of pancreatic islets, and metabolic parameters were examined in rats using reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and other techniques.</p><p><b>RESULTS</b>In insulin-treated diabetic rats, insulin mRNA levels prominently increased by 81.3% (P < 0.05), as compared with untreated diabetic rats. Moreover, timely insulin treatment noticeably improved the insulin content of beta cells, with an increase of 10.2% (P < 0.05), despite a slight reduction in fasting blood glucose (FBG), triglyceride (TG), and free fatty acid (FFA) levels, as compared to an untreated diabetic group.</p><p><b>CONCLUSION</b>Insulin treatment at the onset of T2DM effectively improves insulin synthesis, as confirmed by morphological changes to beta cells in a rat model of type 2 diabetes.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Peso Corporal
/
ARN Mensajero
/
Tejido Adiposo
/
Islotes Pancreáticos
/
Ratas Sprague-Dawley
/
Estreptozocina
/
Diabetes Mellitus Experimental
/
Diabetes Mellitus Tipo 2
/
Quimioterapia
/
Genética
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2004
Tipo del documento:
Artículo
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