Chemokine-like factor 1, a novel cytokine, contributes to airway damage, remodeling and pulmonary fibrosis / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1123-1129, 2004.
Article
en En
| WPRIM
| ID: wpr-291966
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Chemokine-like factor 1 (CKLF1) was recently identified as a novel cytokine. The full-length CKLF1 cDNA contains 530 bp encoding 99 amino acid residues with a CC motif similar to that of other CC family chemokines. Recombinant CKLF1 exhibits chemotactic activity on leucocytes and stimulates proliferation of murine skeletal muscle cells. We questioned whether CKLF1 could be involved in the pathogenesis of inflammation and proliferation in the lung. Therefore we used efficient in vivo gene delivery method to investigate the biological effect of CKLF1 in the murine lung.</p><p><b>METHODS</b>CKLF1-expressing plasmid, pCDI-CKLF1, was constructed and injected into the skeletal muscles followed by electroporation. Lung tissues were obtained at the end of week 1, 2, 3 and 4 respectively after injection. The pathological changes in the lungs were observed by light microscope.</p><p><b>RESULTS</b>A single intramuscular injection of CKLF1 plasmid DNA into BALB/c mice caused dramatic pathological changes in the lungs of treated mice. These changes included peribronchial leukocyte infiltration, epithelial shedding, collagen deposition, proliferation of bronchial smooth muscle cells and fibrosis of the lung.</p><p><b>CONCLUSIONS</b>The sustained morphological abnormalities of the bronchial and bronchiolar wall, the acute pneumonitis and interstitial pulmonary fibrosis induced by CKLF1 were similar to phenomena observed in chronic persistent asthma, acute respiratory distress syndrome and severe acute respiratory syndrome. These data suggest that CKLF1 may play an important role in the pathogenesis of these important diseases and the study also implies that gene electro-transfer in vivo could serve as a valuable approach for evaluating the function of a novel gene in animals.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Patología
/
Fisiología
/
Plásmidos
/
Fibrosis Pulmonar
/
Líquido del Lavado Bronquioalveolar
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Movimiento Celular
/
Electroporación
/
Quimiocinas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Chinese Medical Journal
Año:
2004
Tipo del documento:
Article