Influence of endothelial cells on the proliferation of scar-derived fibroblast in hypertropic scar tissue / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the influence of the endothelial cells on the scar-derived fibroblasts in the hypertropic scar tissue.</p><p><b>METHODS</b>The endothelial cell from the human umbilical vein was cultured and its supernatant was then collected. Thereafter, it was mixed with the pre-cultured scar-derived fibroblasts from the hypertropic scar tissue. The fibroblasts were analyzed with a flow cytometer in a MTT-assay method to evaluate the cell proliferation and its division cycle.</p><p><b>RESULTS</b>The endothelial cells were significantly increasing the scar-derived fibroblast proliferation (P < 0.05), compared with the control. The proportion of the fibroblasts in the S-stage of cell cycle was also quite high.</p><p><b>CONCLUSION</b>The endothelial cells may secrete some active products, which may enhance the proliferation of the scar-derived fibroblasts and stimulate the fibroblasts into the S-stage of cell generation cycle.</p>