The experimental study of culture in vitro of fibroblasts seeded onto human amnion extracellular matrix (HA-ECM) / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 229-231, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-292090
ABSTRACT
<p><b>OBJECTIVE</b>The aim of this experiment is to find proper cell carrier for skin tissue engineering.</p><p><b>METHODS</b>Various concentration of fibroblasts were seeded onto HA-ECM and cultured in vitro. The performance of cells' growth, array, adhesion and collagen secretion on HA-ECM was observed with light microscope and transmission electron microscope.</p><p><b>RESULTS</b>The fusiform fibroblasts oriented radiantly or longitudinally and closely packed onto the HA-ECM, they attached firmly and proliferated to confluence on the stromal surface of HA-ECM.</p><p><b>CONCLUSION</b>The optimal cell concentration is 3.5 x 10(6)/ml, HA-ECM is ideal carrier for fibroblasts because of its excellent scaffold and diffusion characteristics. Futhermore, it has the bioactive molecules such as fibronectin and laminin which play an important role in fibroblasts attachment and proliferation on HA-ECM.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Microscopía Electrónica de Rastreo
/
Células Cultivadas
/
Técnicas de Cultivo de Célula
/
Biología Celular
/
Matriz Extracelular
/
Fibroblastos
/
Hematoxilina
/
Amnios
/
Métodos
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Plastic Surgery
Año:
2002
Tipo del documento:
Artículo
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