Effects of microfilaments on the expression of collagenases and tissue inhibitor of metalloproteinases-1 in fibroblasts / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the roles of microfilaments in the expression of collagenase and TIMP-1 in normal and hypertrophic scar.</p><p><b>METHODS</b>Cell culture and Northern blot hybridization methods were used in this study.</p><p><b>RESULTS</b>After the disruption of microfilaments with cytochalasin B, mRNA contents of collagenase and TIMP-1 both increased significantly in normal and hypertrophic scar fibroblasts.</p><p><b>CONCLUSION</b>The result suggest that the microfilament cytoskeleton may regulate the synthesis and degradation of ECM, which may be a mechanism of gene regulation during wound healing.</p>