Transformation of enhanced green fluorescent protein gene in Paecilomyces lilacinus mediated by Agrobacterium tumefaciens / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 630-634, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-292227
ABSTRACT
The main aim of this study was to transform the enhanced green fluorescent protein gene (egfp) into biocontrol fungus Paecilomyces lilacinus strain 9410. We constructed the expression vector pUPNGT of the fusion gene nptII-egfp using pcDNA3.1(-) as a helper plasmid. The egfp gene was then transformed into P. lilacinus strain 9410 via Agrobacterium tumefaciens-mediated transformation. PCR and Southern blotting analysis showed that the egfp gene was integrated into the genomes of the tested transformants and the integration manner was single-copy. The transformants could generate green fluorescence when they were excited by 488 nm blue laser. These results indicated that the egfp gene had been successfully transformed into P. lilacinus 9410 and expressed in the tested transformants. Our work may provide a new approach to assess environmental safety and practical biocontrol efficacy ofP. lilacinus under different conditions.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Transformación Genética
/
Paecilomyces
/
Reacción en Cadena de la Polimerasa
/
Agrobacterium tumefaciens
/
Proteínas Fluorescentes Verdes
/
Genética
/
Metabolismo
/
Métodos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2010
Tipo del documento:
Artículo
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