Preparation and properties of SiO2 tubes immobilized antibody for HCAg detection / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 545-549, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-292239
ABSTRACT
In order to optimize the fabrication of SiO2 tubes immobilized with antibody for hepatitis C virus antigen (HCAg) detection, we formed the activated amino on the surface of SiO2 tubes by using the activation of aminosilane. Then we immobilized the hepatitis C virus (HCV) monoclonal antibody on the surface of SiO2 tubes by using glutaraldehyde as a chemical cross-linker, followed by detecting HCAg. Sequence tests showed that when the SiO2 tubes were treated in 10% (V/V) aminosilane solution and 3% (V/V) glutaraldehyde solution for 3 hours and 2 hours, respectively, the HCV monoclonal antibody had high immobilization efficiency and low nonspecificity, and the HCAg was detected to 1 ng/mL. This experiment can provide principle and experimental data for establishment of HCAg magnetic immunoassay system.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Química
/
Dióxido de Silicio
/
Antígenos de la Hepatitis C
/
Anticuerpos contra la Hepatitis C
/
Alergia e Inmunología
/
Anticuerpos Inmovilizados
/
Anticuerpos Monoclonales
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2010
Tipo del documento:
Artículo
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