Construction of plasmid expression vector for specific peptide of the rubella virus E1 gene / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To construct a recombinant plasmid vector of the RV specific fragment for expressing the specific fragment of RV E1 protein.</p><p><b>METHODS</b>RNA of the RV attenuated live vaccine Wistar RA27/3 strain was extracted and reversely transcribed. The specific fragment of the E1 gene was amplified and the PCR products cloned in the vector pGEX-2T after purification. Positive clones were selected and identified by two-enzyme digestion and sequence analysis.</p><p><b>RESULTS</b>A 330 bp target fragment was successfully cloned, and the sequence of the recombinant plasmid was consistent with the original sequence.</p><p><b>CONCLUSION</b>Successful cloning of the RV El specific fragment and the construction of the recombinant plasmid have laid a foundation for further expressing the recombinant protein.</p>