Inhibitory effect of cucurbitacin E on the proliferation of ovarian cancer cells and its mechanism / 癌症
Chinese Journal of Cancer
;
(12): 20-24, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-292646
ABSTRACT
<p><b>BACKGROUND AND OBJECTIVE</b>There are various biological activities of cucurbitacin E (CuE), including antitumor effect, anti-chemical carcino-genesis, liver protection, and enhancement of the immunity, and so on. This study was to investigate the effect of CuE on proliferation inhibition and apoptosis induction of ovarian cancer ES-2 cells, and to explore the mechanism.</p><p><b>METHODS</b>ES-2 cells were treated with different concentrations of CuE for 24, 48, and 72 h, respectively. Cell proliferation was tested by MTT assay. The morphologic changes and apoptosis were observed under inverted microscope and fluorescent microscope. Cell cycle distribution was evaluated with flow cytometry. The expression of p-STAT3 was determined by Western blot.</p><p><b>RESULTS</b>The number of ES-2 significantly decreased as the concentration of CuE increased or the time prolonged. Flow cytometry analysis showed that the ratio of ES-2 cells treated 1 micromol/L CuE for 24 h increased both in S phase [from (10.55+/-0.91)% to ( 16.31 +/- 4.61) % ] and in G(2)/M phase [from (18.53+/-1.43)% to (58.34 +/- 5.77)%], while decreased in G(1) phase [from (73.13 +/-4.70)% to (23.12 +/- 5.45)%] (P<0.05). The marked morphological changes of cell apoptosis were clearly observed in ES-2 cells treated with CuE. CuE inhibited the STAT3 phosphorylation in ES-2 cell in a dose- dependent manner.</p><p><b>CONCLUSION</b>CuE can inhibit ES-2 proliferation and induce apoptosis and cell cycle arrest, which may be related to the decreased expression of the intracellular STAT3 phosphorylation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neoplasias Ováricas
/
Patología
/
Farmacología
/
Fosforilación
/
Factores de Tiempo
/
Triterpenos
/
Ciclo Celular
/
Apoptosis
/
Línea Celular Tumoral
/
Proliferación Celular
Límite:
Femenino
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Cancer
Año:
2010
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS