Enrichment of breast cancer stem cells using a keratinocyte serum-free medium / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 2934-2936, 2011.
Article
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| WPRIM
| ID: wpr-292776
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Keratinocyte serum-free medium (K-SFM) is a defined medium used to support the growth of primary keratinocytes and embryonic stem cell. The aim of this research was to optimize enrichment of breast cancer stem cells (CSCs) using K-SFM.</p><p><b>METHODS</b>A K-SFM was used to enrich CSCs from two breast cancer cell lines and a primary culture of breast cancer. RPMI-1640 supplemented with 10% fetal calf serum (FCS) was used as a control. CSCs were identified with flow cytometry using CD44(+)/CD24(-) as molecular markers. The expression of a variety of CSC markers (Oct-4, ABCG2, Nanog, N-cadherin, and E-cadherin) was analyzed with real-time PCR.</p><p><b>RESULTS</b>Much higher percentage of CSCs was achieved with K-SFM: 17.3% for MCF-7 cells, 17.4% for SKBR-3, and 20.0% for primary breast cancer culture. Less than 1% CSC was achieved using RPMI-1640 supplemented with 10% FCS. In comparison to the CSCs obtained with RPMI-1640, CSCs in the K-SFM expressed higher levels of Oct-4, ABCG2, Nanog and N-cadherin, and lower level of E-cadherin.</p><p><b>CONCLUSION</b>K-SFM is an optimal culture medium to maintain and to enrich breast CSCs.</p>
Texto completo:
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Índice:
WPRIM
Asunto principal:
Células Madre Neoplásicas
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Cadherinas
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Queratinocitos
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Medio de Cultivo Libre de Suero
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Proteínas de Homeodominio
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Transportadoras de Casetes de Unión a ATP
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Técnicas de Cultivo de Célula
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Biología Celular
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Línea Celular Tumoral
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Factor 3 de Transcripción de Unión a Octámeros
Tipo de estudio:
Prognostic_studies
Límite:
Female
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Humans
Idioma:
En
Revista:
Chinese Medical Journal
Año:
2011
Tipo del documento:
Article