Changes of glucocorticoid receptor mRNA expression in basolateral amygdale-kindled rats / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2622-2627, 2011.
Artículo
en Inglés
| WPRIM
| ID: wpr-292833
ABSTRACT
<p><b>BACKGROUND</b>Glucocorticoid receptor (GR) is believed to be a major factor in brain maturation and in modulation of a series of brain activity. Hippocampal neurons are abundant in glucocorticoid receptor, and there is significant change in GR expression under certain pathological state. Epilepsy is a special pathological state of the central nervous system. This study aimed to explore the role of GR in epilepsy by observing the change and functions of GR in hippocampus with a basolateral amygdale-electrical kindled rat epilepsy model.</p><p><b>METHODS</b>Firstly, we established the basolateral amygdale-electrical kindled rat epilepsy model. Then GR mRNA expression in the hippocampus was assayed by semi-quantitative reverse transcription-PCR in this experiment. In addition, the processes of epileptic seizures were observed and electroencephalograms were recorded. One-way analysis of variance (ANOVA) was employed for comparing means of multiple groups, followed Fisher's least significant difference (LSD) for paired comparison.</p><p><b>RESULTS</b>The rats were successfully kindled after an average of (13.50 ± 3.99) times electrical stimulation, in which it was showed that GR mRNA expression reduced obviously as compared with the control group and the sham groups (P < 0.001). The down-regulation of GR mRNA expression was abated or reversed by some anti-epilepsy drugs (P < 0.001 compared with the epilepsy group), accompanied by attenuation of seizures and improvement of electroencephalograms.</p><p><b>CONCLUSIONS</b>Down-regulation of hippocampal GR mRNA expression may be related to the kindling. Anti-epilepsy drugs exposure can retard this change.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Receptores de Glucocorticoides
/
Ratas Sprague-Dawley
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Epilepsia
/
Genética
/
Amígdala del Cerebelo
/
Excitación Neurológica
/
Metabolismo
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2011
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS