Investigation of the mechanisms of coagulation factor VIIa-induced colon cancer SW620 cell proliferation and migration / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 485-489, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-293083
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanisms that coagulation factor VIIa promotes proliferation and migration of a colon cancer cell line (SW620 cells) in vitro.</p><p><b>METHODS</b>The expression of interleukin 8 (IL-8), tissue factor (TF), caspase-7 and p-p38 MAPK in SW620 cells treated with factor VIIa or protease activated receptor 2 agonist (PAR2-AP) was measured by ELISA, Western-blotting and QT-PCR.</p><p><b>RESULTS</b>Factor VIIa and PAR2-AP induced IL-8 expression at both mRNA and protein levels, upregulated TF mRNA expression and TF activity, but down-regulated caspase-7 mRNA and p-p38 MAPK levels in SW620 cells. The effects of factor VIIa were not only blocked by anti-TF but also by anti-PAR2 antibodies.</p><p><b>CONCLUSION</b>Factor VIIa binds to TF on cell surface, forming a complex which activates PAR2, then provoking IL-8 and TF expression, and suppresses caspase-7 expression, thus promotes the tumor cell proliferation and migration. p38 MAPK may negatively regulate this process.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oligopéptidos
/
Patología
/
Farmacología
/
ARN Mensajero
/
Tromboplastina
/
Factor VIIa
/
Movimiento Celular
/
Interleucina-8
/
Neoplasias del Colon
/
Línea Celular Tumoral
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2009
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS