Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 618-623, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-293262
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the possible mechanism through which Artemisinin induced apoptosis in pancreatic cell line.</p><p><b>METHODS</b>Column chromatography, thin layer chromatography (TLC) and proton NMR spectroscopy were used to purify Artemisinin. The flowcytometry was employed to detect apoptosis and reactive oxygen species (ROS).</p><p><b>RESULTS</b>The results indicated that 50% inhibiting concentration (IC50 value) for pancreatic cell line (RIN) was 45 μmol/L of Artemisinin. Artemisinin had no cytotoxic effect on the growth of peripheral blood lymphocytes. The mechanism of apoptosis was evaluated by measuring intracellular ROS. It was shown that Artemisinin-induced apoptosis occurred independently of the binding of CD95L to CD95 receptor in the RIN cells. Moreover, Artemisinin, in a dose-dependent manner, could significantly increase the level of ROS.</p><p><b>CONCLUSION</b>Artemisinin can induce apoptosis in the RIN cells via the generation of ROS and triggering the intrinsic pathway of cell death.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neoplasias Pancreáticas
/
Patología
/
Farmacología
/
Propidio
/
Factores de Tiempo
/
Especies Reactivas de Oxígeno
/
Apoptosis
/
Anexina A5
/
Colorimetría
/
Receptor fas
Límite:
Humanos
Idioma:
Inglés
Revista:
Chinese journal of integrative medicine
Año:
2014
Tipo del documento:
Artículo
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