Effect of antibody-targeted chemotherapy with pingyangmycin on prostate cancer cells in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 406-408, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-293365
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of antibody-targeted chemotherapy against human prostate cancer LNCaP cells in vitro.</p><p><b>METHODS</b>The monoclonal antibody 7E11C5.3 against human prostate cancer was conjugated to pingyangmycin (PYM), mediated by dextran T-40, and the immunoreactivity of 7E11C5.3 was determined by indirect enzyme-linked immunosorbent assay. The bacteriostatic activity of the conjugate was determined using TTC assay, and its cytotoxicity against LNCaP cells was determined by MTT assay.</p><p><b>RESULTS</b>The 7E11C5.3PYM molar ratio was l54 in the conjugate, and the immunoreactivity of 7E11C5.3 was decreased by approximately 10% to 20% after conjugation. The bacteriostatic activity of conjugated PYM was 25% of that of free PYM. The 50% inhibitory doses (IC50) of 7E11C5.3-PYM conjugate and free PYM against the in vitro cultured LNCaP cells were 9.41-/+1.98 microg/ml and 29.92-/+7.88 microg/ml, respectively.</p><p><b>CONCLUSION</b>7E11C5.3-PYM conjugate displays stronger cytotoxicity against anti-prostate cancer effects than free PYM.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Neoplasias de la Próstata
/
Bleomicina
/
Supervivencia Celular
/
Sistemas de Liberación de Medicamentos
/
Inmunoconjugados
/
Citotoxicidad Inmunológica
/
Línea Celular Tumoral
/
Alergia e Inmunología
Límite:
Humanos
/
Masculino
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2008
Tipo del documento:
Artículo
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