Analysis of mitochondrial DNA D-loop region mutation and its significance in human oncocytoma / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 767-770, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-293486
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mutation in mitochondrial DNA displacement-loop (mtDNA D-loop) region in oncocytoma and its relationship with tumorigenesis and tumor development.</p><p><b>METHODS</b>The mtDNA D-Loop region of 20 thyroid or renal oncocytomas and the adjacent normal tissues were amplified by PCR, and then sequenced. Five human fetal renal tissues were collected as matched controls.</p><p><b>RESULTS</b>Among the 20 oncocytomas, 21 mutations which focused on hypervariable region I (HVI) were found in 7 tumor tissues and 1 normal tissue with the mutation rates of 35% and 5%, respectively. At the same time, 191 polymorphisms were found in the 20 cases.</p><p><b>CONCLUSION</b>mtDNA D-loop region, especially HV I, is the mutational hotspot of oncocytomas, which may be closely related with mtDNA duplicating rate and the function of mitochondria.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Polimorfismo Genético
/
ADN Mitocondrial
/
Neoplasias de la Tiroides
/
Análisis Mutacional de ADN
/
Secuencia de Bases
/
Adenoma Oxifílico
/
Genética
/
Neoplasias Renales
/
Mitocondrias
/
Mutación
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2010
Tipo del documento:
Artículo
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