Development and identification of monoclonal antibodies of cape jasmine proteins in Reduning injection / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1606-1609, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-294059
ABSTRACT
Liposoluble cape jasmine proteins were used to immunize BALB/C mice. Indirect ELISA was utilized to develop one monoclonal antibody by integrating SP2/0 cells and spleen cells from immunized BALB/C mice. The subclass of the monoclonal antibody was identified as IgG2b, with Kappa chain as its light chain. The ascite titer of 2H8 monoclonal antibody was 1204 080. Western-blot analysis proved that 2H8 reacted with cape jasmine proteins to identify specific liposoluble protein with molecuar weight of around 58.5 kDa. Dot-ELISA was established with 2H8 ascites as the primary antibody, showing the minimum detectable amount of 19.5 ng. This study lays a foundation for the development of protein kits of Reduning injection.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas de Plantas
/
Juego de Reactivos para Diagnóstico
/
Ensayo de Inmunoadsorción Enzimática
/
Medicamentos Herbarios Chinos
/
Hipersensibilidad a las Drogas
/
Alergia e Inmunología
/
Métodos
/
Ratones Endogámicos BALB C
/
Anticuerpos Monoclonales
/
Especificidad de Anticuerpos
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2013
Tipo del documento:
Artículo
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