Reg IV, a differentially expressed gene in colorectal adenoma / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 918-922, 2003.
Artículo
en Inglés
| WPRIM
| ID: wpr-294203
ABSTRACT
<p><b>OBJECTIVE</b>To discover and identify differentially expressed genes associated with colorectal adenoma formation and the role of RegIV in colorectal adenoma differentiation.</p><p><b>METHODS</b>A subtracted cDNA library was constructed with cDNAs that were isolated from either the normal mucosa or adenoma tissue of a single patient. Suppressive subtractive hybridization (SSH) combined with virtual northern blotting was used to characterize differentially expressed genes and contigs were assembled by electronic cloning (in silico cloning) with the EST database. Semi-quantitative RT-PCR was performed in 9 colorectal adenomas.</p><p><b>RESULTS</b>The amino acid sequence was determined with open reading frame (ORF) prediction software and was found to be 100% homologous to the protein product of RegIV (a novel gene isolated from a large inflammatory bowel disease library). RegIV was found to be highly expressed in all of the adenoma samples (9/9) compared with the normal mucosa samples, while 5/6 cases showed RegIV to be more strongly expressed in adenocarcinoma.</p><p><b>CONCLUSION</b>RegIV may play an important role in the initiation of colorectal adenoma differentiation, and its detection may be useful in the early diagnosis of colorectal adenoma formation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Pronóstico
/
Neoplasias Colorrectales
/
Adenoma
/
Northern Blotting
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Lectinas Tipo C
/
Proteínas Asociadas a Pancreatitis
/
Genética
/
Metabolismo
/
Hibridación de Ácido Nucleico
Tipo de estudio:
Estudio pronóstico
/
Estudio de tamizaje
Límite:
Humanos
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2003
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS