Molecular subtyping of Vibrio cholerae isolates by pulsed-field gel electrophoresis in Shenzhen from 1993 / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 491-494, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-294307
ABSTRACT
<p><b>OBJECTIVE</b>To determine the genetic relationships between different Vibrio cholerae isolates in Shenzhen from 1993 to 2002.</p><p><b>METHODS</b>Chromosomal DNA from 60 isolates was digested in seakem gold agrose with restriction enzyme Not I and plugs were then analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis (PFGE) patterns of V. cholerae isolates were clustered using BioNumerics software.</p><p><b>RESULTS</b>39 distinctive PFGE patterns were identified with each pattern having 20 to 30 bands. Most PFGE patterns were divided into cluster A or cluster B.</p><p><b>CONCLUSION</b>The closely related pandemic clone clusters of V. cholerae strains did exist in Shenzhen. PFGE of V. cholerae could be used for active surveillance and tracking for cholerae.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Filogenia
/
Vibrio cholerae
/
China
/
Cólera
/
Epidemiología
/
Clasificación
/
Electroforesis en Gel de Campo Pulsado
/
Genética
/
Métodos
/
Microbiología
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Chinese Journal of Epidemiology
Año:
2007
Tipo del documento:
Artículo
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