Cloning and expression of extracellular region gene located in N-terminus of Leishmania Donovani / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 820-824, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-294561
ABSTRACT
The objective of this study was to construct and express recombinant prokaryotic plasmid pET32a (+)- ast1 in E. coli BL21(DE3). Amastin gene was amplified from genomic DNA of Leishmania Donovani and its transmembran region was predicted by the methods of SOSUI and Tmpred; astl located in N-terminus of amastin gene was amplified and cloned into prokaryotic plasmid pET32a(+), which was named pET32a(+)-ast1, and then rAST1 was expressed in E. coli BL21(DE3). The results of SDS-PAGE and immunobloting assay showed that a fusion protein rAST1 (relative molecular mass about 27 kDa) was able to express in BL21. The recombinant prokaryotic plasmid pET32a(+)- ast1 was successfully constructed, and noted to be efficiently expressed in E. coli BL21(DE3).
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plásmidos
/
Leishmania donovani
/
Proteínas Recombinantes de Fusión
/
Proteínas Protozoarias
/
Clonación Molecular
/
Genes Protozoarios
/
Escherichia coli
/
Espacio Extracelular
/
Genética
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Biomedical Engineering
Año:
2009
Tipo del documento:
Artículo
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