Phage display peptide library for screening the peptides that specifically bind to osteoblasts cells / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 438-441, 2015.
Article
en Zh
| WPRIM
| ID: wpr-294687
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the experimental methods that the phage peptide library technology screening human osteoblast specificity polypeptide, which will provide the basis of the experiment of the Ti surface biolization modification.</p><p><b>METHODS</b>Human calvarial osteoblasts were used as the target cells for whole-cell biopanning from a 12-mer peptide phage-display library. Cell eluent and cell lysis buffer were cultivate and count respectively after washing. Then the target cells were analyzed by enzyme-linked immunosorbent assay (ELISA) and immunofluorescence detection to authenticate the positive phage clones by human gingival fibroblast as the absorber cells. The positive phage clones were deduced by DNA sequencing.</p><p><b>RESULTS</b>After four rounds of screening, twenty-two positive phage clones were found out from randomly selected phage monoclonals, whose single-strand DNA were extracted and sequenced. Amino acid sequence of the highest frequency peptide was MGWSWWPETWPM.</p><p><b>CONCLUSIONS</b>The specific peptide against human osteoblasts can be obtained from a phage-display peptide library for use as a new research approach and experimental basis of the biolization modification of the titanium surface.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Osteoblastos
/
Péptidos
/
Titanio
/
Ensayo de Inmunoadsorción Enzimática
/
Secuencia de Bases
/
Técnica del Anticuerpo Fluorescente
/
Secuencia de Aminoácidos
/
Análisis de Secuencia de ADN
/
Biblioteca de Péptidos
/
Análisis de Secuencia de Proteína
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Stomatology
Año:
2015
Tipo del documento:
Article