A component of earthworm fibrinolytic enzyme having higher thrombolytic activity than total components in vivo / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1068-1073, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-294887
ABSTRACT
<p><b>AIM</b>To select higher thrombolytic and lower toxic single component of earthworm fibrinolytic enzymes (EFE).</p><p><b>METHODS</b>EFE containing total components were obtained by affinity chromatography from Eisenia fetida. Using ion-exchange chromatography to separate three main components EfP-0-2, EfP-I-1 and EfP-I-2 from EFE, their thrombolytic activity and toxicity were compared with EFE.</p><p><b>RESULTS</b>Among these components, EfP-I-1 had higher thrombolytic activity in vitro. When 4.5 mg x kg(-1) of these components were injected, the contents of fibrinogen in rat serum were not affected, but only EfP-I-1 exhibited distinct thrombolytic activity. When 6.0 mg x kg(-1) of them were injected intravenously, the bleeding time was not evidently delayed only by EfP-I-1. The acute toxicity test showed that the LD50 of EfP-I-1 was higher than EFE by 2. 17 times.</p><p><b>CONCLUSION</b>Because of distinct thrombolytic activity, lower toxicity in vivo, higher content in EFE and easy to purify, EfP-I-1 was adapted to be developed as a single component medicine for treating thrombus.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oligoquetos
/
Farmacología
/
Espectrofotometría Infrarroja
/
Tiempo de Sangría
/
Sangre
/
Fibrinógeno
/
Datos de Secuencia Molecular
/
Química
/
Secuencia de Aminoácidos
/
Ratas Wistar
Límite:
Animales
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2006
Tipo del documento:
Artículo
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