Identification of hydroxylate metabolites of daidzein and its sulfate conjugates in rat urine by LC-ESI/MS(n) / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1004-1009, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-294898
ABSTRACT
<p><b>AIM</b>To identify the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine.</p><p><b>METHODS</b>Urine samples from 0 - 24 h were collected after single ig dose of 500 mg x kg(-1) daidzein to each of six rats. The urine samples were purified by SPE column (SPE C18) and analyzed with liquid chromatographic-tandem electrospray ionization ion trap mass spectrometry (LC-ESI/MS(n)) for potential metabolites.</p><p><b>RESULTS</b>Several new hydroxylate metabolites and its sulfate conjugates were found and identified in rat urine.</p><p><b>CONCLUSION</b>LC-ESI/MS(n) is proved to be a simple, rapid, sensitive and specific technique for identification of the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Semillas
/
Glycine max
/
Sulfatos
/
Orina
/
Estructura Molecular
/
Química
/
Reproducibilidad de los Resultados
/
Cromatografía Liquida
/
Ratas Sprague-Dawley
/
Espectrometría de Masa por Ionización de Electrospray
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2006
Tipo del documento:
Artículo
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