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Liquid chromatography-tandem electrospray ionization ion trap mass spectrometric assay for the metabolites of jatrorrhizine in rat urine / 药学学报
Acta Pharmaceutica Sinica ; (12): 846-851, 2006.
Artículo en Chino | WPRIM | ID: wpr-294927
ABSTRACT
<p><b>AIM</b>To identify the main metabolites of jatrorrhizine in rat urine.</p><p><b>METHODS</b>The rat urine samples were collected 0 - 72 h after ig 12 mg x kg(-1) jatrorrhizine, then the samples were purified through C18 solid-phase extraction cartridge. The purified samples were analyzed by combining liquid chromatography and tandem electrospray ionization ion trap mass spectrometry (LC-ESI/ITMS(n)). Identification and structural elucidation of the metabolites were performed by comparing the changes in molecular masses, retention-times and full scan MS(n) spectra with those of the parent drug.</p><p><b>RESULTS</b>At least seven phase I metabolites (such as de-methyl, de-hydrogen and hydroxyl metabolites) and eleven phase II metabolites (such as glucuronide conjugates and methyl-conjugates) were identified in rat urine.</p><p><b>CONCLUSION</b>The developed LC-ESI/ITMS(n) method is not only simple and rapid but also sensitive and specific for the identification of metabolites of jatrorrhizine in rat urine.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Plantas Medicinales / Orina / Berberina / Estructura Molecular / Química / Cromatografía Líquida de Alta Presión / Ratas Wistar / Espectrometría de Masa por Ionización de Electrospray / Coptis / Metabolismo Límite: Animales Idioma: Chino Revista: Acta Pharmaceutica Sinica Año: 2006 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Plantas Medicinales / Orina / Berberina / Estructura Molecular / Química / Cromatografía Líquida de Alta Presión / Ratas Wistar / Espectrometría de Masa por Ionización de Electrospray / Coptis / Metabolismo Límite: Animales Idioma: Chino Revista: Acta Pharmaceutica Sinica Año: 2006 Tipo del documento: Artículo