Expression of human papillomavirus type 16/18 in human cervical carcinomas by the quantum dot fluorescent in-situ hybridization / 中华病理学杂志
Chinese Journal of Pathology
; (12): 675-677, 2010.
Article
en Zh
| WPRIM
| ID: wpr-295155
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate fluorescence in situ hybridization labeled with quantum dots (QDs) for the detection of human papillomavirus 16/18 (HPV16/18) infection in cervical carcinoma patients.</p><p><b>METHODS</b>A total of 80 biopsy samples of squamous carcinoma of cervix were assayed for HPV 16/18 infection by using quantum dot labeled fluorescent in situ hybridization (QD-FISH) and chromogenic in situ hybridization (CISH) techniques, respectively. The results obtained by using two different methods were statistically analyzed.</p><p><b>RESULTS</b>The positive rate for HPV16/18 by QD-FISH was 88.8% (71/80), higher than that (80.0%) by CISH, however, the result was statistically not significant (P=0.127). The positive detection rates for HPV16/18 by using both methods increased coincidentally with raising of the tumor grading stage.</p><p><b>CONCLUSION</b>The sensitivity and specificity of HPV infection detectable by QD-FISH is higher than that by the CISH technique.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Patología
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Virología
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Carcinoma de Células Escamosas
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Displasia del Cuello del Útero
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Neoplasias del Cuello Uterino
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Compuestos Cromogénicos
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Sensibilidad y Especificidad
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Hibridación Fluorescente in Situ
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Infecciones por Papillomavirus
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Puntos Cuánticos
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
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Female
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Año:
2010
Tipo del documento:
Article