Profile of chromosomal imbalances in 10 cases of primary alveolar rhabdomyosarcoma analyzed by comparative genomic hybridization / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 571-576, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-295246
ABSTRACT
<p><b>OBJECTIVE</b>The aim of this study was to characterize the profile of chromosomal imbalances of alveolar rhabdomyosarcoma (ARMS).</p><p><b>METHODS</b>One-step RT-PCR was used to detect the expression of PAX3-FKHR and PAX7-FKHR fusion transcripts in 10 cases of alveolar rhabdomyosarcoma and in an ARMS cell line. Comparative genomic hybridization (CGH) was used to investigate the genomic imbalances in these cases. It was analyzed according to the histological type, pathologic grading, clinical staging, gender and age, respectively.</p><p><b>RESULTS</b>The 10 patients with alveolar rhabdomyosarcoma showed evidence of increased or decreased DNA sequence copy numbers involving one or more regions of the genome. (1) The frequently gained chromosome arms of ARMS were 12q, 2p, 6p, 6q, 10q, 2q, 4q, 15q, 1p, 9q, 14q and 18q (> or = 30.0%), and the frequently lost chromosome arms of ARMS were 3p, 6p, 20q and 21q (> 30.0%). (2) The frequently gained chromosome arm translocation associated with ARMS were 12q, 10q, 2p, 2q, 6p, 6q, 1p, 4q, 8q, 11q, 14q and 15q (> 30.0%). The frequently lost chromosome arms were 3p, 5q, 6p, 1q, 8p, 11p, 20q and 21q (> 30.0%). (3) There were no correlation between chromosome changes and histological type, pathologic grade, clinical stage, gender and age, respectively.</p><p><b>CONCLUSION</b>These observations suggest that (1) 12q, 2p, 6p, 6q, 10q, 2q, 4q, 15q, 1p, 9q, 14q, 18q gain and 3p, 6p, 20q, 21q loss may correlated with ARMS-related carcinogenesis; (2) 12q gain may be correlated with translocation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Cromosomas Humanos Par 3
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Cromosomas Humanos Par 5
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Cromosomas Humanos Par 6
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Cromosomas Humanos Par 12
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Proteínas de Fusión Oncogénica
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Aberraciones Cromosómicas
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Deleción Cromosómica
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Rabdomiosarcoma Alveolar
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Línea Celular Tumoral
Límite:
Adolescente
/
Adulto
/
Anciano
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Niño
/
Femenino
/
Humanos
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Lactante
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Masculino
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2009
Tipo del documento:
Artículo
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