Analysis of allele frequencies of HLA-DRB1*12:01:01G and HLA-DRB1*14:01:01G groups / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To discriminate and analyze the relative frequencies of alleles in HLA-DRB1*120101G(HLA-DRB1*120101/1206/1210/1217) and HLA-DRB1*140101G (DRB1*140101/1454) groups and assess their associations with HLA-DRB3 and HLA-DQB1 loci.</p><p><b>METHODS</b>A total of 115 DNA samples previously typed as HLA-DRB1*120101G and 108 samples from HLA-DRB1*140101G were selected. DNA sequences for exons 1 to 3 of the HLA-DRB1 locus were analyzed for HLA-DRB1*120101G, and exons 2 to 3 were analyzed for HLA-DRB1*140101G by polymerase chain reaction sequence-based typing (PCR-SBT). Genotyping of HLA-DRB3 and HLA-DQB1 were achieved by PCR-SBT.</p><p><b>RESULTS</b>Among 115 samples previously typed as HLA-DRB1*120101G, 101 (87.8%) were confirmed as HLA-DRB1*120101 and 14 (12.2%) were HLA-DRB1*1210, but HLA-DRB1*1206 and HLA-DRB1*1217 alleles were not identified. For 108 samples previously typed as HLA-DRB1*140101G, all were typed as HLA-DRB1*1454. HLA-DRB1*120101 was linked with HLA-DRB3*010102 and HLA-DQB1*0301, while HLA-DRB1*1210 was strongly linked with HLA-DRB3*020201 and HLA-DQB1*0301. HLA-DRB1*1454 was strongly linked with HLA-DRB3*020201 and two different HLA-DQB1*0502, *0503 alleles.</p><p><b>CONCLUSION</b>HLA-DRB1*120101 was more prevalent than HLA-DRB1*1210 in the HLA-DRB1*120101G group, and HLA-DRB1*1454 was the dominant allele for HLA-DRB1*140101G.</p>