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Fluorescence in situ hybridization study of acute myeloid leukemia with cryptic chromosome rearrangements / 中华医学遗传学杂志
Chinese Journal of Medical Genetics ; (6): 690-693, 2011.
Artículo en Chino | WPRIM | ID: wpr-295552
ABSTRACT
<p><b>OBJECTIVE</b>To detect specific chromosome rearrangements in acute myeloid leukemia (AML) using interphase-fluorescence in situ hybridization (FISH).</p><p><b>METHODS</b>All cases were studied by R-band karyotypic analysis using direct method and/or short-term culture for chromosomes preparation. Interphase-FISH was performed in 108 cases of AML with M5, M4, M2, M3 subtypes including 103 cases with normal karyotypes, 4 cases with chromosomal abnormalities other than specific chromosomal rearrangements using chromosome translocation probe such as AML1/ETO, PML/RARα, CBFβ/MYH11 and MLL.</p><p><b>RESULTS</b>Of 38 cases of M2-AML without t(8;21) on conventional cytogenetics(CC) analysis, 4 cases showed positivity for AML1/ETO fusion transcript, which included 2 cases with typical signal model and 2 with insertion. Of 9 cases of M3-AML without t(15;17) on CC analysis, 6 showed positivity for PML/RARα fusion transcript including 2 with typical signal model, 3 with insertion, one without PML/RARα rearrangement on reverse transcription-PCR and FISH assay using PML/RARα probe. FISH assay using the RARα dual color, break-apart rearrangement probe indicated a partial deletion of RARα. Of 23 cases with M4 or M4EO-AML without inv(16) on CC analysis, 3 showed positivity for CBFβ/MYH11 fusion transcript. Of 38 cases without 11q23 translocation on CC analysis, all cases were negative for MLL rearrangement.</p><p><b>CONCLUSION</b>Interphase-FISH can detect specific chromosome rearrangements such as AML1/ETO, PML/RARα or CBFβ/MYH11 in some AML cases with normal karyotype, though it seemed less useful for the detection of MLL rearrangement.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Translocación Genética / Leucemia Mieloide Aguda / Proteínas de Fusión Oncogénica / Bandeo Cromosómico / Hibridación Fluorescente in Situ / Diagnóstico / Genética / Cariotipificación / Métodos Tipo de estudio: Estudio diagnóstico Límite: Adolescente / Adulto / Femenino / Humanos / Masculino Idioma: Chino Revista: Chinese Journal of Medical Genetics Año: 2011 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Translocación Genética / Leucemia Mieloide Aguda / Proteínas de Fusión Oncogénica / Bandeo Cromosómico / Hibridación Fluorescente in Situ / Diagnóstico / Genética / Cariotipificación / Métodos Tipo de estudio: Estudio diagnóstico Límite: Adolescente / Adulto / Femenino / Humanos / Masculino Idioma: Chino Revista: Chinese Journal of Medical Genetics Año: 2011 Tipo del documento: Artículo