Study on the mimic epitopes screening program severe acute respiratory syndromes antigen with random phage peptide library / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 904-906, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-295624
ABSTRACT
<p><b>OBJECTIVE</b>To screen the severe acute respiratory syndromes (SARS) mimotopes with random phage peptide library and to investigate their immunogenicity.</p><p><b>METHODS</b>Using SARS sera as selective molecule, a 12 mer phage peptide library was biopanned and positive clones containing the mimic epitopes were selected. The immuno-characteriation of the epitopes were then investigated.</p><p><b>RESULTS</b>2 positive clones that having specific affinity to SARS sera were obtained. The DNA sequencing data showed no homology between the sequences of the deduced amino acid of the two mimic antigen peptides and the sequence of SARS.</p><p><b>CONCLUSION</b>SARS mimotopes were obtained by phage peptide library screening. This method might provide a new approach for SARS therapy and vaccine development.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Secuencia de Bases
/
Clonación Molecular
/
Análisis de Secuencia de ADN
/
Biblioteca de Péptidos
/
Síndrome Respiratorio Agudo Grave
/
Materiales Biomiméticos
/
Alergia e Inmunología
/
Genética
/
Epítopos
Tipo de estudio:
Ensayo Clínico Controlado
/
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Epidemiology
Año:
2005
Tipo del documento:
Artículo
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